ABSTRACT
Abstract Background l-Asparaginase is essential in the treatment of childhood acute lymphoblastic leukemia. If immunoglobulin G anti-l-asparaginase antibodies develop, they can lead to faster plasma clearance and reduced efficiency as well as to hypersensitivity reactions, in which immunoglobulin E can also participate. This study investigated the presence of immunoglobulin G and immunoglobulin E anti-l-asparaginase antibodies and their clinical associations. Methods Under 16-year-old patients at diagnosis of B-cell acute lymphoblastic leukemia confirmed by flow cytometry and treated with a uniform l-asparaginase and chemotherapy protocol were studied. Immunoglobulin G anti-l-asparaginase antibodies were measured using an enzyme-linked immunosorbent assay. Intradermal and prick skin testing was performed to establish the presence of specific immunoglobulin E anti-l-asparaginase antibodies in vivo. Statistical analysis was used to investigate associations of these antibodies with relevant clinical events and outcomes. Results Fifty-one children were studied with 42 (82.35%) having anti-l-asparaginase antibodies. In this group immunoglobulin G antibodies alone were documented in 10 (23.8%) compared to immunoglobulin E alone in 18 (42.8%) patients. Immunoglobulin G together with immunoglobulin E were simultaneously present in 14 patients. Children who produced exclusively immunoglobulin G or no antibodies had a lower event-free survival (p-value = 0.024). Eighteen children (35.3%) relapsed with five of nine of this group who had negative skin tests suffering additional relapses (range: 2-4), compared to none of the nine children who relapsed who had positive skin tests (p-value < 0.001). Conclusion Children with acute lymphoblastic leukemia and isolated immunoglobulin G anti-l-asparaginase antibodies had a higher relapse rate, whereas no additional relapses developed in children with immunoglobulin E anti-l-asparaginase antibodies after the first relapse.
Subject(s)
Asparaginase , Immunoglobulin E , Immunoglobulin G , Escherichia coli , Precursor Cell Lymphoblastic Leukemia-Lymphoma , Antibodies, Neutralizing , HypersensitivityABSTRACT
La respuesta inflamatoria de las vías aéreas superiores, ocasionada por infecciones virales y/o bacterianas, constituye la causa más común de consulta médica y la rinosinusitis una complicación frecuente. Las alteraciones en los mecanismos de inmunidad local y sistémica son factores que favorecen el desarrollo de la rinosinusitis. La participación de mediadores derivados del eosinófilo es también un factor importante en la fisiopatología de la inflamación de la mucosa nasal
Subject(s)
Humans , Bacteria/pathogenicity , Eosinophils/immunology , Immune System/immunology , Nasal Mucosa/immunology , Nasal Mucosa/physiopathology , Paranasal Sinuses/immunology , Paranasal Sinuses/physiopathology , Respiratory Tract Diseases/immunology , Respiratory Tract Diseases/physiopathology , Rhinitis/physiopathology , Sinusitis/physiopathology , Viruses/pathogenicityABSTRACT
Se estudiaron 75 pacientes para evaluar el uso de aire caliente en pacientes con rinitis alérgica. El rango de edad de los pacientes fue de 10 a 69 años con un media de 32.8. Los resultados preliminares de este estudio demostraron que con la inhalación de aire caliente, hay un aumento de la resistencia nasal de 0.215 a los 3 y 5 minutos posteriores, sin variar a las 2 horas P < 0.05. La función mucociliar se midión por la prueba de sacarina, se encontró una correlación significativa entre los dos grupos de pacientes, con aire caliente y con aire normal gama = .972, pero sin ser significativo P > 0.01. La citología nasal no demostró anomalías morfológicas en el grupo estudiado